Ion Exchange Chromatography – Optimisation and Robustness Analysis Using Design of Experiments
نویسنده
چکیده
Proteins are often separated using ion-exchange chromatography. This method can however show poor resolution if not carefully optimised. This work aims to find all the parameters that affect the separation as well as determining which are critical. By using Design of Experiment the optimum separation is found and a robustness analysis is conducted. Polyclonal antibody Immunoglobulin G (IgG) and Bovine Serum Albumin (BSA) are separated on a strong anionexchange column. The parameters focused on are the elution gradient, pH, sample concentration, flow rate and sample size.
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